Isolation and Molecular Characterization of Sea Ice Microbial Communities of East Antarctica.

Access URI: http://npdc.ncaor.gov.in/npdc/search_mf_data.action?search=freeText&expedition_type=all&parameter=MF638031375&userType=user&freeTextValue=Isolation+and+Molecular+Characterization+of+Sea+Ice+Microbial+Communities+of+East+Antarctica

Brief Description (Abstract):

During the XXI Indian Antarctic Expedition bacterial samples have been collected from different habitats of Antarctica. A total of 150 pure isolates were obtained by streak plate method. Of these, 64 isolates are marine. Morphological, biochemical, physicochemical and molecular characterization of the all the strains have been completed. Studies on the salt and temperature tolerance of the marine isolates indicate that all the strains are halotolerant psychrotrophs. Studies on the pH tolerance revealed that they are neutrophils and tolerated a pH range of 4 to 11.8. PCR based RFLP analysis of various strains indicate that there are 13 genetically different marine species. Partial sequencing of 16 S rRNA of all the RFLP differentiated strains have been completed. Blasting and species identification will be possible only after sequencing the complete 1538 bp gene, which is in progress.

Status: Completed

Purpose: The present investigation an attempt has been made to study the biotechnological applications of Antarctic microbes in general and Sea Ice Microbial Communities in particular and the preliminary results obtained are presented here.

Presentation Format: Documentdigital

Distribution Format: unknown

Distribution Format Version: unknown

Supplemental Information:

TableDigital

Keyword(s): Earth Science-Biological Classification,

Place/Location(s): Maitri, Antarctic,

Project: Isolation and Molecular Characterization of Sea Ice Microbial Communities of East Antarctica,

Topic Category(s): Biota,

Date(s): 2020-09-30 (Creation),

Resource Contact(s):
1. Name : Dr. V Wilsanand
    Role : PrincipalInvestigator
    Phone : 8322525515
    E-Mail : ictd [ at ] ncpor.res.in
    Organization Name : National Centre for Polar and Ocean Research (NCPOR)
    Address : Headland Sada, Vasco da Gama, Goa 403804
    City : Vasco-da-Gama
    State : Goa
    Postal Code : 403804
    Country : IND

Distributor Contact(s):
1. Name : Mr. Sakthivel Samy V
    Role : Distributor
    Phone : +91-832-2525515
    E-Mail : vssamy [ at ] ncpor.res.in
    Organization Name : National Centre for Polar and Ocean Research (NCPOR)
    Address : Headland Sada, Vasco da Gama, Goa 403804
    City : Vasco-da-Gama
    State : Goa
    Postal Code : 403804
    Country : IND

Metadata Contact(s):
1. Name : Mr. Sakthivel Samy V
    Role : PointOfContact
    Phone : +91-832-2525515
    E-Mail : vssamy [ at ] ncpor.res.in
    Organization Name : National Centre for Polar and Ocean Research (NCPOR)
    Address : Headland Sada, Vasco da Gama, Goa 403804
    City : Vasco-da-Gama
    State : Goa
    Postal Code : 403804
    Country : IND

Extent:
Start Date : 2001-01-01
End Date   : 2002-12-31