8cba19d9-8743-4f4c-aa05-f7d06e9feade English UTF-8 Dataset Mr. Sakthivel Samy V National Centre for Polar and Ocean Research (NCPOR) +91-832-2525515 Headland Sada, Vasco da Gama, Goa 403804 Vasco-da-Gama Goa 403804 IND vssamy@ncpor.res.in Point of contact 2020-12-11 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Isolation and Molecular Characterization of Sea Ice Microbial Communities of East Antarctica. 2020-09-30 Creation Document digital During the XXI Indian Antarctic Expedition bacterial samples have been collected from different habitats of Antarctica. A total of 150 pure isolates were obtained by streak plate method. Of these, 64 isolates are marine. Morphological, biochemical, physicochemical and molecular characterization of the all the strains have been completed. Studies on the salt and temperature tolerance of the marine isolates indicate that all the strains are halotolerant psychrotrophs. Studies on the pH tolerance revealed that they are neutrophils and tolerated a pH range of 4 to 11.8. PCR based RFLP analysis of various strains indicate that there are 13 genetically different marine species. Partial sequencing of 16 S rRNA of all the RFLP differentiated strains have been completed. Blasting and species identification will be possible only after sequencing the complete 1538 bp gene, which is in progress. The present investigation an attempt has been made to study the biotechnological applications of Antarctic microbes in general and Sea Ice Microbial Communities in particular and the preliminary results obtained are presented here. Completed Dr. V Wilsanand National Centre for Polar and Ocean Research (NCPOR) 8322525515 Headland Sada, Vasco da Gama, Goa 403804 Vasco-da-Gama Goa 403804 IND ictd@ncpor.res.in Principal investigator Earth Science-Biological Classification Theme Maitri Antarctic Place Isolation and Molecular Characterization of Sea Ice Microbial Communities of East Antarctica Project English biota 20.0 140.0 -90.0 30.0 2001-01-01 2002-12-31 TableDigital unknown unknown Mr. Sakthivel Samy V National Centre for Polar and Ocean Research (NCPOR) +91-832-2525515 Headland Sada, Vasco da Gama, Goa 403804 Vasco-da-Gama Goa 403804 IND vssamy@ncpor.res.in Distributor http://npdc.ncaor.gov.in/npdc/search_mf_data.action?search=freeText&expedition_type=all&parameter=MF638031375&userType=user&freeTextValue=Isolation+and+Molecular+Characterization+of+Sea+Ice+Microbial+Communities+of+East+Antarctica