c3f0b79e-4e59-4b6f-9ea0-995aab515d4a English UTF-8 Dataset Mr. Sakthivel Samy V National Centre for Polar and Ocean Research (NCPOR) +91-832-2525515 Headland Sada, Vasco da Gama, Goa 403804 Vasco-da-Gama Goa 403804 IND vssamy@ncpor.res.in Point of contact 2020-12-11 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Recording Aerobic Culturable Microbial Load of Soil Core Samples from Larsemann Hills, East Antarctica. 2020-10-12 Creation Document digital Larsemann Hills is an ice-free area of around 40 square km, located in Princess Elizabeth Land, East Antarctica. In this study, five soil cores were collected from five different locations in Larsemann Hills. A great variation of four major types of bacterial colonies, based on their size and colour, was found and recorded; when the soil suspension was plated both onto Nutrient Agar and Yeast Extract Agar media. These microbes were grown in different media and different temperatures. Their morphology was examined by Scanning Electron Microscopic and growth pattern at different temperature ranging from 4 ?C to 37 ?C was also recorded. The samples showed a great variability, although collected from almost same geographical region. In this study, five soil cores were collected from five different locations in Larsemann Hills. For collection, sterilized steel borers were used, with a diameter of 1 cm and length of 8 cm. Hammer was used to push the borer into the soil. Steel piston of compatible diameter was used for pushing the soil out of the borer into sterilized cryovials of 5 ml volume. The borer and piston were washed and burned every time, before collecting soil from a new location, to eliminate any chance of contamination. In every location air temperature, soil temperature, and the coordinates were recorded. After collection, the samples were stored in ?24 ?C in the refrigerator. Completed Dr. Soumya Biswas National Centre for Polar and Ocean Research (NCPOR) 8322525515 Headland Sada, Vasco da Gama, Goa 403804 Vasco-da-Gama Goa 403804 IND ictd@ncpor.res.in Principal investigator Earth Science-Land Surface Theme Larsemann Hill Antarctic Place Recording Aerobic Culturable Microbial Load of Soil Core Samples from Larsemann Hills Project English 20.0 140.0 -90.0 30.0 2007-01-01 2008-12-31 TableDigital unknown unknown Mr. Sakthivel Samy V National Centre for Polar and Ocean Research (NCPOR) +91-832-2525515 Headland Sada, Vasco da Gama, Goa 403804 Vasco-da-Gama Goa 403804 IND vssamy@ncpor.res.in Distributor http://npdc.ncpor.res.in/npdc/search_mf_data.action?search=freeText&expedition_type=all&parameter=MF-836910266&userType=user&freeTextValue=Recording+Aerobic+Culturable+Microbial+Load+of+Soil+Core+Samples+from+Larsemann+Hills%2C+East+Antarctica.